@Article{MachadoLobMarCorCor:2015:AnCeAd,
               author = "Machado, M. M. and Lobo, A. O. and Marciano, F. R. and Corat, 
                         Evaldo Jos{\'e} and Corat, M. A. F.",
          affiliation = "{Universidade do Vale do Para{\'{\i}}ba (UNIVAP)} and 
                         {Universidade do Vale do Para{\'{\i}}ba (UNIVAP)} and 
                         {Universidade do Vale do Para{\'{\i}}ba (UNIVAP)} and {Instituto 
                         Nacional de Pesquisas Espaciais (INPE)} and {Universidade de 
                         Campinas (UNICAMP)}",
                title = "Analysis of cellular adhesion on superhydrophobic and 
                         superhydrophilic vertically aligned carbon nanotube scaffolds",
              journal = "Materials Science and Engineering C",
                 year = "2015",
               volume = "48",
                pages = "365--371",
             keywords = "Carbon nanotube, Cell adhesion, Embryonic fibroblasts, Green 
                         fluorescent protein, Superhydrophilicity, Transgenic mice.",
             abstract = "We analyzed GFP cells after 24 h cultivated on superhydrophilic 
                         vertically aligned carbon nanotube scaffolds. We produced two 
                         different densities of VACNT scaffolds on Ti using Ni or Fe 
                         catalysts. A simple and fast oxygen plasma treatment promoted the 
                         superhydrophilicity of them. We used five different substrates, 
                         such as: as-grown VACNT produced using Ni as catalyst (Ni), 
                         as-grown VACNT produced using Fe as catalyst (Fe), VACNT-O 
                         produced using Ni as catalyst (NiO), VACNT-O produced using Fe as 
                         catalyst (FeO) and Ti (control). The 
                         4\′,6-diamidino-2-phenylindole reagent nuclei stained the 
                         adherent cells cultivated on five different analyzed scaffolds. We 
                         used fluorescence microscopy for image collect, ImageJ® to count 
                         adhered cell and GraphPad Prism 5® for statistical analysis. We 
                         demonstrated in crescent order: Fe, Ni, NiO, FeO and Ti scaffolds 
                         that had an improved cellular adhesion. Oxygen treatment 
                         associated to high VACNT density (group FeO) presented 
                         significantly superior cell adhesion up to 24 h. However, they do 
                         not show significant differences compared with Ti substrates 
                         (control). We demonstrated that all the analyzed substrates were 
                         nontoxic. Also, we proposed that the density and hydrophilicity 
                         influenced the cell adhesion behavior.",
                  doi = "10.1016/j.msec.2014.11.06",
                  url = "http://dx.doi.org/10.1016/j.msec.2014.11.06",
                 issn = "0928-4931",
             language = "en",
           targetfile = "analysis of cellular.pdf",
        urlaccessdate = "27 abr. 2024"
}